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Composite

Part:BBa_K1660001:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-07)


rMpL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 208
    Illegal AgeI site found at 304
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We searched the cDNA of MpL, and optimized the codon for E. coli.


Source

It comes from Macrolepiota procera.

References